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Construction of Reversed Caspase-3 Protein and Its Effect on Apoptosis Induction in SKBr3 Cells
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Abstract:
Objective: To investigate the apoptosis induction effect due to the expression of reversed caspase-3 gene in SKBr3 cells.Methods: Reversed caspase-3 gene was gain by recombinant PCR and cloned into expression vector pcDNA3 to transfected SKBr3 cells. The expression and the apoptosis induction effect of reversed caspase-3 gene on SKBr3 cells were observed by HE and immunohistochemical staining. The cell cycle of transfected SKBr3 cells were analyzed by FCM. The tumor suppression effect of reversed caspase-3 gene was observed through administrating recombinant plasmids to BALB/c nude mouses bearing SKBr3 tumor.Results: Reversed caspase-3 gene can be expressed in SKBr3 stably. Reversed caspased-3 protein can induce obvious apoptosis in vitro and inhibit tumor growth in vivo.Conclusion: Reversed caspase-3 gene can effectively induce SKBr3 cells to apoptosis.
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