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Induction of Apoptosis in Melanoma by FasL Gene Transfer in vitro
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Abstract:
Objective: To evaluate effects of Fas ligand (FasL) gene transfer in vitro for apoptosis of malignant melanoma. Methods: We constructed recombinant adenoviral vector containing human FasL cDNA(Ad-FL) under the control of a cytomegalovirus (CMV) promoter, which encodes human Fas ligand and transfected into two melanoma cells. Flow cytometric analysis, RT-PCR identify the expression of Fas/FasL. TUNEL, fluorescence microscope were used to analyse apoptosis. Results: Two melanoma cells surface express Fas, whereas not express FasL detected by RT-PCR and flow cytometric analysis; while tumor cells transducted by Ad-FL can express high level of FasL. Ad-FL can induce two melanoma cells apoptosis or suppress their growth in vitro. Conclusion: Recombinant adenovirus FasL had great effect on inducing the apoptosis of human melanoma in vitro.
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