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Effect of Tiam-1 Antisense Oligodeoxynucleotides (ASODNS)on Antimetastasis of Tumor
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Abstract:
Objective： Tiam-1 antisense gene was used to downregulate Tiam-1 mRNA and protein expression, thus tumor metastasis could be suppressed. Methods：In this study Tiam-1 mRNA was examined by RT-PCR. The expression of Rho protein was examined by flow cytometry. Basement membrane was used to examine the effect of Tiam 1 antisense gene on metastatic behavior of PG cells. Results：The relative expression level of Tiam-1 mRNA was reduced after exposure to Tiam-1 ASODNS-Lf. Only 72 hours of exposure to Tiam-1 ASODNS, cell metastasis of treated group was inhibitted significantly. The invasive cell numbers through matrigel reduced from 945±40 to 649±35 (P<005). Tiam-1 ASODNS-Lf significantly inhibitted Rho protein expression ratio from 27.86% to 16.86%(P<0.001), which related to signal transduction pathway. Conclusions：Antisense gene inhibits invasion and metastasis of tumor cells through a cascade reaction of gene-protein-cell effect and provides the theoretical and experimental bases for clinical therapy and study of antimetastasis.
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