Carcino-Embryonic Antigen Promotor Controls the Specific Cytotoxicity of Ecoli Cytosine Deaminase Gene in Colorectal Carcinoma Cells in vitro

doi:10.3872/j.issn.1007-385X.2003.1.002

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Archive > Volume 10 Issue 1 > 2003,10(1):5-8. DOI:10.3872/j.issn.1007-385X.2003.1.002 Prev Next

Carcino-Embryonic Antigen Promotor Controls the Specific Cytotoxicity of Ecoli Cytosine Deaminase Gene in Colorectal Carcinoma Cells in vitro

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Abstract:

Objective: To investigate whether carcino-embryonic antigen(CEA) promotor determines the specific expression of E. coli. cytotosine deaminase(EC-CD) gene and the specific cytoxicity in CEA-producing colorectal carcinoma cells. Methods:Two recombinant adenovirus vectors, AdCEACD containing EC-CD gene controlled under CEA promotor and AdCMVCD containing cytomegalovirus(CMV) promotor and EC-CD gene, were constructed. The expression of EC-CD gene in cells was tested with RT-PCR and cytotoxic effects were assayed with MTT method. Results：The CEA-producing cells (human colorectal carcinoma cell line Lovo cell) showed EC-CD mRNA expression and became sensitive to 5-fluorocytosine (5-FC) after infection with AdCEACD and AdCMVCD respectively, The CEA-nonproducing cells(Hela cell) expressed EC-CD mRNA and were sensitive to 5-FC after infection with AdCMVCD, but had no expression of EC CD mRNA and no cytotoxic effect after infection with AdCEACD. Conclusion：CEA promotor could control the expression of EC-CD gene in CEA-positive colorectal carcinoma cells specifically in vitro.

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R735.35 Q782

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