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Recombinant Kringle 5 of Human Plasminogen for Mammary Cancer Gene Therapy Mediated by Adenovirus
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Abstract:
Objective: To investigate the inhibitory effects of Ad-K5 on the proliferation of endothelial cells and the growth of mammary cancer.Methods: Kringle 5 of human plasminogen was constructed by PCR, and then cloned to plasmid pCA13. Recombinant adenovirus Ad-K5 was obtained through homologous recombination in 293 cells. The inhibition effect of Ad-K5 on cell proliferation was observed in vitro. The tumor size was measured at different intervals to observe the antitumoral effect of Ad-K5 in vivo.Results:The mRNA expression of K5 gene was detected in Ad-K5 infected B-Cap-37 cells. Ad-K5 inhibited the growth of ECV-304 cells, but had almost no effects on B-Cap-37 cells. It seemed that Ad-K5 could inhibit endothelial cells but not kill the cancer cells directly. Ad-K5 could also inhibit the tumor growth in vivo.Conclusion: Ad-K5 could inhibit the growth of solid mammary tumor.
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Clc Number:
R737.9
