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Specific Inhibition of Hepatocarcinoma Cells by Recombinant Adenovirus Expressing Melittin
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Abstract:
Objective:To evaluate the inhititory effects of the recombinant adenovirus vector expressing melittin gene under the control of human α-fetoprotein (AFP).Methods:The melittin gene was inserted into adenoviral vector through a bacterial homologous recombinant system, then the recombinant adenoviral vector was transfected to 293 cells to generate the desired recombinant adenoviruses. AFP-positive or AFP-negative hepatocarcinoma cells or hepatic cells were infected respectively with the recombinant adenoviruses and the proliferative inhibition was evaluated by MTT method.Results:Recombinant adenovirus containing melittin gene and AFP promoter was constructed successfully. When infected with the recombinant adenoviruses the inhibition of proliferative was observed in AFP-positive hepatocarcinoma cells, but not in AFP-negative hepatocarcinoma cells. Conclusions: Recombinant adenovirus expressing melittin gene under the control of human AFP promoter could specifically inhibit the proliferation of AFP-positive hepatocarcinoma cells.
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Clc Number:
R735.7
