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The Characterization of NK-92 Cell Line Transfected with Human Stem Cell Factor Gene
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Abstract:
Objective:To study the characterization of NK cell line transfected with hSCF gene.Methods:pcDNA3-hSCF was transfected into NK-92 cell line with LipofectAMINE ,RT-PCR was used to identify NK-92-hSCF cell which express hSCF and the activity was assayed by TF-1 cell line. CD3, CD16 and CD56 molecules were tested by FACS. Results: We established NK-92-hSCF cell line which express hSCF steadily, and its proliferation increased compared with parental cell line when incubated with rhIL-15 or rhIL-2. Conclusion: The characterization of NK-92 could be changed by transfecting with hSCF gene, and it′s proliferation was improved, The gene transfection of NK-92 cell made it suitable for clinical applications.
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Clc Number:
R392.12
