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Induction of Osteosarcoma Anoikis Apoptosis by Human Interferon-α
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Abstract:
Objective:Survived from Anoikis is one important reason for maliganant tumor performing matastasis and local invasion. The present study focused on the mechanism and signal transduction of osteosarcoma anoikis induced by human interferon (IFN) α2a.Methods:Interferon-α2a induced anoikis was detected on a non-attachment culture model. The osteosarcoma anoikis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick and labeling (TUNEL) method. Osteosarcoma cells apoptosis and integrin receptors expression were detected by flow cytometry. The cysteine aspartate-specific proteases (caspase) signal transduction was detected by cleavage of synthethic substractes. Results: Human IFN-α2a induced the anoikis apoptosis of osteosarcoma cell MG- 63, with concomitant upregulation of caspase-8 and caspase-3 activity. The expression of integrin receptor α and αv was not influenced evidently.Conclusion: The present results suggested that human IFN-α2a decreased the metastasis of osteosarcoma by inducing the anoikis of osteosarcoma cell MG- 63. Our results also indicated that caspase signal transduction might regulate this anoikis apoptosis in MG- 63.
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Clc Number:
R737.33
