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The Effects of Recombinant Adenovirus-Mediated Wild Type p53 cDNA on Human Colorectal Cancer Cell Lines with Different p53 Status
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Abstract:
Objective:To explore the inhibition effects of ectogenic wild-type p53 cDNA(Ad-wtp53) on colorectal carcinoma cell lines with different p53 gene status and search for the role of wild type p53 tumor suppressor gene in occurrenc and progress of malignant tumor.Methods:MTT process was taken to choose optimal transfection titre. Three kinds of cell lines(p53 gene deletion, mutation and nomal) were transferred by Ad-wtp53 in optimal titre. The inhibition effects of these cell lines were observed and compared. Results:The best titre is 1000 MOI and p53 gene deletion cell line (THC-8908) shew the highest sensitivity. G1-S transition period blocking effects occurred in all cell lines and G2-M phase regulation effects were not coincidence in three colorectal cell lines. Conclusions:Recombinant adenovirus-mediated wild type p53 gene observably inhibited colorectal carcinoma cell lines growth and proliferation, blocked cell cycle in G0/G1 phase and displayed obvious different actions on G2-M phase among cell lines with different p53 status.
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Clc Number:
R735.2
