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All Trans Retinoid Acid Inhibit Cell Growth in Human Retinoblastoma Cells Via Phosphorylation of JNK
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Abstract:
Objective: To investigate all trans retinoid Acid (ATRA) inhibition of cell growth in human retinoblastoma Y79 cells, and its mechanism. Methods:Antiproliferation effects of ATRA on Y79 cells were determined by 3H-thymidine incorporation. Cell cycle analysis was performed by flow cytometry. JNK phosphorylation was analyzed by Western blot analysis.Results: After 36 h treatment with 10-6mol·L-1 ATRA, 3H-thymidine incorporation decreased to 40%, under the same condition, Y79 cells were arrested in G0/G1 and Sub-G1 peak appeared. Curcumin, JNK blocker, blocked the growth inhibition by ATRA. JNK was phosphorylated in 10 to 20 min. Conclusion: JNK-phosphorylating mediated ATRA inhibition of apoptosis in Y79 cells. These results suggested that ATRA might have clinical application for treatment of retinoblastoma.
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Clc Number:
R735.34
