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Therapeutic Effects and Apoptosis Mechanism of bcl-xs Gene to the Human Ovarian Carcinoma
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Abstract:
Objective：To investigate the effects and mechanism of adenovirus-bcl-xs gene on the inhibition of ascites tumor cells and survival rate of nude mice transplanted intraperitoneally with human ovarian carcinoma. Methods：By using of a reproduced adenovirus-bcl-xs gene infected in NUTU-19 cells, we transfered it intraperitoneally to ascites tumor model of human ovarian carcinoma transplanted in nude mice, detected the ascites formation, the survival time and survival rate of nude mice with the human ascites tumor. The weight and toxic-adverse systemically effects of nude mice were observed and the gene expression was detected by immunocellchemistry. The apoptotic cells were quantitatively determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL).Results：The adenovirus-bcl-xs gene had inhibitory potential on NUTU-19 cells. bcl-xs protein was expressed and apoptotic cells were observed. The survival time of nude mice was longer and the survival rate was higher, and the time of ascites formation was retarted. Conclusions：The results suggested that the transfer of adenovirus-bcl-xs gene to the ascites tumour of nude mice with human ovarian carcinoma could improve the survival rate of nude mice and retard the time of ascites formation. It may be a useful method of gene therapy in the treatment of ovarian carcinoma.
Keywords:
adenovirus-bcl-xs gene ; human ovarian carcinoma ; nude mice model ; ascites tumor ; gene therapy ; cell apoptosis
Clc Number:
R730.5 R739.7
