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Synergistic Inhibition of TNP-470 and Recombinant Human Endostatin on the Growth of Mice Lung Adenocarcinoma LA795
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Abstract:
Objective:To investigate the combined inhibitive effect of TNP-470 and rhES on the growth of lung adenocarcinoma LA795 in T739 mice. Methods: The purified rhES was acquired by using methanol to induce the recombinant pichia pastoris. GS115 and heparin affinity chromatography. The T739 mice inoculated with LA795 cells were randomized into three groups, 10 mice per group, one group was injected with PBS for 14 days, the other two groups were respectively treated with rhES and TNP-470+rhES. To observe the tumor growth in different groups, and the tumor volume was measured with caliper. The microvessel density(MVD) of tumors were measured by using immunohistochemistry. Results: The purified rhES was acquired. In compared with PBS group, the tumor growth of other two groups was inhibited significantly. And the tumor volume of TNP-470+rhES group are smaller than the rhES group (P<0.01). immunohistochemistry showed the combined therapy has more inhibitory effect on angiogenesis. Conclusion: The combined treatment with TNP-470 and rhES showed more than additive effect in tumor growth inhibition when compared with treatment with individual antiangiostatic protein rhES.
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