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Therapeuti Effects of NDV HN Gene and CAV VP3 Gene on BALB/c Nude Mice Bearing HCT Tumor
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Abstract:
Objective: The Explore the effective method of tumor gene therapy and studying the antitumor effect of NDV HN gene and apoptin VP3 gene.Methods: The carcinoma of colon model in BALB/c nude mice was constructed and the pVHN and pVVP3 DNA-liposome complexes were injected into the tumor. The therapeutic effect of NDV HN gene and apoptin VP3 gene to BALB/c nude mice bearing HCT tumor was observed. and the expressions of the Bcl-2 and PCNA of the tumors were detected with immunohistochemistry. Results: Compared with the control group ,the tumors in the group of combination therapy were decreased obviously,and the rate of suppressing tumor reached 70 percent. The results of pathological tissue section showed that apoptosis was induced greatly in the group of combination therapy,and the tumor cells in some range disappeared.The results of immunohistochemistry showed that the Bcl-2 and PCNA protein were expressed in control group and all of therapy groups , the Bcl-2 expression rates of control group and the therapy group with pVHN were marked difference (P<0.05) and the PCNA expression rates of control group and the therapy group with pVHN and pVVP3 were very marked difference (P<0.01) .Conclusion: NDV HN gene and apoptin VP3 gene are able to suppress the proliferation of the tumor cells in BALB/c nude mice bearing HCT tumor and induce apoptosis of the tumor cells. The apoptosis that induced by NDV HN gene and apoptin VP3 gene may correlated with down-regulation of Bcl-2 protein.
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