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The Antitumor Effect of the Nucleic Vaccine Constructed with Newcastle Disease Virus HN Gene
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Abstract:
Objective:To investigate the role of HN in NDV antitumor. Method: Nucleic recombinant plasmid pVHN was constructed with pVAX1 and NDV HN gene and transfected into OS732 cells in vitro. 72 h after transfection, OS732 cells were collected and the content of cell surface sialic acid was measured. 6 weeks old BALB/c mice were implanted with S180 sarcoma through the injection of 2×10 6 S180 cells. On 7th day and 17th day after tumor transplantation,100 μg pVHN recombinant plasmid was administered intratumorly in vivo respectively. Control group was treated with pVAX1 as the same method. Serum of BALB/c mice bearing S180 sarcoma was separated and measured the content of sialic acid. T lymphocyte subsets were detected by FACS combined with rat anti mouse CD3+, CD4+, CD8+ antibodies. Results:pVHN was constructed correctly and expressed in OS732 cell. pVHN reduced the surface sialic acid of OS732 cell significantly in vitro. Expression of pVHN in vivo augmented CD8+ T lymphocyte subset and decreased serum sialic acid content. Conclusion: HN gene plays an important role in the reduction of sialic acid and the augmentation of CD8+ T lymphocyte subset
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