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Quantitative Detection of spp1 Expression in Lung Carcinoma
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Abstract:
Objective: To quantitatively detect the expression level of spp1 gene in lung carcinoma. Method: The RT-PCR and real-time fluorescence quantitative RT-PCR method was used. Result: The spp1 gene expressed higher in tumor tissue than that in normal tissue in all 24 cases of lung carcinoma. In 9 cases spp1 expression level was about or more than 100 times as that of normal lung tissue. Conclusion: The spp1 gene expressed higher in lung carcinoma tissue than that of normal lung tissue and indicated that it might be an important marker in the diagnosis of the lung cancer.
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