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Antitumor Immunity of CD4+ T Cells Subset in CIKs
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Abstract:
Objective:To observe antitumor immunity of CD4+ T cells subset in CIKs.Methods: After large scale of amplification in vitro, CD4+ T cells subset in CIKs was isolated by magnetic beads separation columns. Distribution of Th1/ Th2 in CD4+ T cells subset in CIKs was analysized by intracellular cytokine staining. Cytotoxicity of purified CD4+ T cells subset in CIKs against raji cells and apoptosis of raji cells after 4 h and 20 h coculture were determined by LDH method and fluorescent staining method.Results: Purity of enriched CD4+ T cells subset in CIKs reached 96%. Comparing with PBMCs, significant increase in Th1 subset and Th0 subset were observed but no statistical differences were found in Th2 subset. Few raji cells were lysed by CD4+ T cells subset in CIKs after 4 h co-incubation. But after 20 h co-incubation, the same effective lysis of raji cells as CD4- T cells subset was obtained in CD4+ T cells subset in CIKs. Fluorescent staining showed that CD4+ T cells subset in CIKs induced apoptosis of raji after 4 h coculture. Conclusion: The present study suggested that CD4+ T cells in CIKs were not only regulatory cells capable of modulating host immune system, but also immune effectors capable of inducing apoptosis in tumor cells.
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