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Anti-Tumor Mechanism of Anti-CD20 Antibody in vitro: B Lymphoma Cell Apoptosis Induced by Chimeric Antibody Fragment Fab′
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Abstract:
Objective:To study anti-tumor mechanism of chimeric anti-CD20 antibody fragment Fab′ in vitro. Methods：The effect of anti-CD20 antibody fragment Fab′ on Raji cells growth was observed using MTT; Annexin V-FITC and PI were used to assay Raji cells apoptosis induced by Fab′; bcl-2 gene expression level change in Raji cells induced by Fab′ was assayed using RT-PCR and Western blotting. Results：The result of MTT indicated that the growth of Raji cells was inhibited by chimeric anti-CD20 antibody fragment Fab′ (IC50, 24.2 μg/ml); bcl-2 gene expression level in Raji cells was decreased prominently by Fab′. Conclusions： It is relative between growth inhibition of Raji cells and apoptosis induced by chimeric anti-CD20 antibody fragment Fab′, and bcl-2 level decline was involved.
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