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The Biological Characterestics of Dendritic Cells Derived from Peripheral Blood of Lung Cancer Patients
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Abstract:
Objective：To study the biological characteristics of dendritic cells (DC) derived from peripheral blood of patients with lung cancer.Methods：Peripheral blood mononuclear cells (PBMC) were isolated from 11 lung cancer patients and healthy donors respectively, cultured with GM-CSF (100 μg/L) and IL-4 (50 μg/L). After pulsed with apoptotic lung cancer cells for 24 h, TNF-α（10 μg/L）added or agonist CD40mAb (5 mg/L) for a further induction of 3～4 days. Phenotypes of DC were detected by FCM. The capacities for DC to uptake antigens were monitored at day 7 and day 11 detected by FITC-dextran uptake test.3H-thymidine incorporation test was used to measure the proliferative capability of T cells. Results： Both DC derived from PBMC of lung cancer patients and from PBMC of healthy donors showed high expressions of CD1α, CD80, CD83, CD86 and HLA-DR, which were known as associated markers of mature DC. The 7th day DC derived from lung cancer patients PBMC showed a strong capacity to uptake antigens. DC were induced by TNF-α or CD40mAb, then became mature. DC entirely lost their capacities of uptake antigens, while DC could stimulate autologous and allogenic T cells' proliferation. When matured DC co-incubated with autologous T cells for 48 h, activated T cells could be observed and the number of activated T cells was significantly increased (P<0.05).Conclusion：The functional and mature DC could be induced from peripheral blood of lung cancer patients in vitro.
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