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The Therapentic Effects of Ets-1 Antisense Oligoxydeonucleotide On Gastric Carcinoma
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Abstract:
Objective: To investigate the therapeutic effects of ets-1 antisense oligoxydeonucleotide(AsODN) on gastric carcinoma. Methods: Cultured SGC-7901 cells were devided into control group, sense oligoxydeonucleotide(sODN) group and AsODN group. After transfection for 24 h, expression of ets-1mRNA was detected by RT-PCR, growth inhibition was detected by colone formation assay, in vitro invasive ability assay was carried out in Transwell chamber，the animal model of xenotransplanted gastric carcinoma in nude mice was established to detect invasive ability in vivo. Results: ets-1 AsODN could under-regulate the expression of ets-1 mRNA, number of colone formation of AsODN group was significantly lower than the other two groups(24.2±4.8 vs 47.6±8.1 vs 44.3±7.6，P<0.01）, so was the number of cells which crossed to the lower surface of the matrigel-coated filters (97.1±11.1 vs 198.7±18.3, 205.8±22.2， P<001）. ets-1 AsODN could also inhibit the growth of xenotransplanted gastric carcinoma in nude mice significantly. Conclusion: ets-1 AsODN had therapeutic effects on gastric cancinoma, and the inhibition of the invasive ability of gastric cancer cells might be one of its mechanisms.
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