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Influence of AMB on Immunosuppression of S180 Supernatant
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Abstract:
Objective:To investigate the effects of AMB(Astragalus Mongholicus Bge) on the immunosuppression of tumor cell line S180 supernatant.Methods:The effects of the supernatant of S180 cultured with or without AMB on the transformation, IL-2 production and NK activity of murine splenocytes and the reactivity of CTLL-2 to IL-2 were measured by MTT, and the effect on the IL-2Rα expression of murine splenocyte was detected by flow cytometry. Results: The supernatant of S180 alone could strongly inhibit the five tests. Adding AMB to the superanant of S180,the results of the five tests were up-regulated substantially.The superanants of S180 which were cultured first in AMB for 24 h or 48 h,then washing off AMB or not,and recultured for 24 h remarkably up-regulated the five tests. Conclusions:AMB could reverse the immunosuppressions of the supernatant of S180,down-regulate the synthesis and/or secretion of immunosuppressive subsance by tumor cells.
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