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Anti-Tumor Effects of Anti-hCGβ Antibody Induced by Gene Immunization
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Abstract:
Objective：To investigate the anti-tumor effects of anti-hCGβ antibody induced by gene immunization with ectopic human chorionic gonadotropin β-subunit. Methods：Sera were collected at the indicated times from the mice immunized with plasmid TR421-ehCGβ-coding for hCGβ and mock DNA respectively and were determined the levels of anti-hCGβ antibody by ELISA. The tumor cells expressing ehCGβ were treated with different doses of sera, in which complement were or not inactivated. The proliferation and morphological change as well as apoptosis of tumor cells were detected by \[3H\]-Thymidine incorporation assay, converse-microscope and FACS, respectively. Results：All mice immunized with plasmid TR421-ehCGβ developed high levels of anti-hCGβ antibody, which could inhibit obviously the proliferation of Hela cells compared with the serum from animals immunized with mock DNA (P<0.05), even if complement was inactivated. However, the inhibition of normal serum decreased significantly after complement was inactivated. The inhibition activity of the anti-hCGβ serum correlated with the expression level of ehCGβ in the tumor cells. The number of dead Hela cells increased obviously upon being incubated with the anti-hCGβ serum, while the percentage of the apoptotic cells was only 3.42%, which had no significant difference with the cells incubated with the normal serum (1.88%). Conclusion：The anti-hCGβ antibody induced by gene immunization had significant inhibitory influence on the proliferation of tumor cells expression ehCGβ in complement-independent manner.
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