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Tumor Associated Antigen Encoding Gene CHP2 Inhibited Cell Proliferation
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Abstract:
Objective: To study the effect of cell proliferation transfected with tumor associated antigen encoding gene CHP2. Methods: Using PCR method to clone CHP2 encoding gene and inserting it into the eukaryotic expression vector pcDNA3 to construct the recombination pcDNA-CHP2. The fusion gene was identified by enzyme digestion and DNA sequencing. 293 cells transfected CHP2 genes were selected by G418 pressure and the cell proliferation was measured by 3-TdR uptake. Results: Identified by enzyme digestion and sequencing, CHP2 gene was correctly cloned into pcDNA3. The results of the western blot showed the transfected cell could express CHP2 protein. 3-TdR uptake value of the CHP2 transfected cell was lower than the control. Conclusions: CHP2 could inhibit cell proliferation and it maybe an important regulator involved in cell proliferation.
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