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Method of Biological Activity Evaluation of Recombinant Human neu Epitope Peptide 12
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Abstract:
Objective:To establish a sensitive and effective bioassay method for in vitro evaluation of biological activity of recombinant human neu epitope peptide 12. Methods:Mice were used in the test. By comparing results of antibody for an ELISA in different species of mouse, including dosage and production intervals of immunization and parameters, an in vitro bioassay protocol was formulated. Results:According to dose-response curve of recombinant human neu epitope peptide 12 on FVB transgenic mice(TgN MMTVneu 202 Mul，Jackson Lab.，USA), the optimal reaction time and dosage were determined. The results were accurate, and objective, and had good reproducibility, CV% is 6.7% (n=4); the percentage of positive mice were used for biological activity evaluation of recombinant human neu epitope peptide 12. Conclusion:The established method of using FVB transgenic mice to test the recombinant human neu epitope peptide 12 bioactivity can be used in routine control of the biological activity evaluation of recombinant human neu epitope peptide 12.
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