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The Expression of mdr-1 and mrp Genes in the Human Breast Cancer Cells, and the Effects of Arscenic Trioxide on the Gene Expression
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Abstract:
Objective: To investigate the expression of mdr-1 and mrp genes and observe the effects of arsenic trioxide (As2O3) on the gene expression.Methods: The doze of cytotoxicity and non-cytotoxicity of As2O3 was examined through MTT assay. The expressions of mdr-1 and mrp genes were studied by RT-PCR method.Results: The non-cytotoxic dose of As2O3 was 0.2 μmol/L and the low cytotoxic dose was 0.8 μmol/L to MCF-7/ADM cell line(P<0.05). Expression of mdr-1 and mrp genes was positive in MCF-7/ADM cells, and the expression of mdr-1 was stronger than the mrp gene, then the expression of mdr-1 and mrp genes was negative in the MCF-7/S cells; Arsenic trioxide could down-regulate the expression of mdr-1 and mrp genes in MCF-7/ADM and the lowcytotoxic dose of As2O3 had more strong effect than the non-cytotoxic dose of As2O3. Conclusions:The strong expressions of mdr-1 and mrp genes were responsible for the induced resistance of MCF-7/ADM cells to ADM; Arsenic trioxide reverses partly the multidrug resistance of the MCF-7/ADM cells with more kinds of biologic mechanisms.
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