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Inhibitory Effect of Gastric Cancer by Adenoviral Transduction of Mouse Endostatin Gene
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Abstract:
Objective：To study the inhibitory effects on gastric cancer by adenoviral transduction of mouse endostatin gene.Methods：Mouse endostatin gene was cloned into the genome of replication-defective adenovirus specific for the tumor cells by virus recombination technology, and then its biological activities were surveyed in vitro. Animal model of gastric cancer bearing nude mice were established to assay the expression of mouse endostatin and inhibition for tumor cell in vivo. Results：To construct the recombinant adenovirus vector pCA13-mEndo and explore its expression in the levels of both mRNA and protein. The protein appearing the anti-angiogenesis activity was tested by chicken chorio-allantoic membrane assay. It can also inhibit tumor to grow and induce tumor cell to apoptosis significantly in vivo of animal. Conclusions:The recombination adenovirus can express biologically active mEndostatin effectively, which results in inhibiting the growth of micro-blood vessels and proliferation slowly. This lays the foundation for the angiogenesis therapy of the solid tumor in clinical application.
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Clc Number:
R730.23
