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The Therapeutic Effects of AFP Gene-Modified Dendritic Cell-Based Tumor Vaccine by Different Re-Infused Pathways
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Abstract:
bjective:To observe the anti-tumor effects of adenovirus carring AFP gene transfected DC using different inculabe administrations.Methods:The immunotherapy effects of AFP-DC vaccine were compared among administration of intra-vein, hypo-dermal and intra-tumor. The protective and therapeutic effects of AFP-DC tumor vaccine were detected by 4 h 51 Cr releasing assay and depleting T cells and NK cells. Results: The therapentic effect of Hypo-dermal was significantly better than assays of the other two ways in inhibiting tumor growth and prolonging the survival period of the mice(P<0.05). AFP-DC tumor vaccine could more effectively induced specific CTL cytotoxicity, protective response, and resistance to the rechallenge of tumor cells. Both CD4+ and CD8+T cells were required for AFP-DC tumor vaccine to induce antitumor immunological rejection. In the effective phase, antitumor immunological rejections were mainly depended on CD8+T cells, and CD4+ T cells were not necessary. Blocking NK cells in the induction and effect phase didn′t have obvious influence on the antitumor immunity, suggesting that NK cells were not required for antitumor immunity induced by AFP-DC tumor vaccine. Conclusion: AFP-DC tumor vaccine by hypo-dermal administration could induce vaccine more efficient antitumor immune responses than intravein or intra-tumor ways in vivo, which provided new ways to the immunotherapy of tumor mediated by DC.
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Clc Number:
R737.11
