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The Antitumor Effects of Tumor Vaccine with Expression of Granulocyte-Macrophage Colony-Stimulating Factor Anchored on Mouse Melanoma Tumor Cell Surface
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Abstract:
Objective:To investigate the vaccine potency of GM-CSF anchored B16 tumor cells. Methods:In this study, mGM-CSF was expressed on surface of B16 mice melanoma cells by GPI-modifying. C57BL/6 mice were inoculated with GM-CSF anchored cells and wide-type B16 cells to evaluate whether the GM-CSF anchored cells could elicit a protective and systemtic antitumor response. Results:GM-CSF anchored cells resulted in remarkable loss of tumorgenicity in syngenetic mice. The tumor occurrence rate of GM-CSF anchored B16 cells was 58.8% on C57BL/6 mice with 1×10 6- B16 cells/mice inoculated (n=12) and that of wide-type B16 cells was 100%, The C57BL/6 mice receiving inoculation with 5×10 5 GM-CSF anchored cells/mice never grew tumor. These mice were challenged with wide-type B16 cells, and only a minority of mice grew tumor after wide-type B16 cells inoculation. Conclusion: GM-CSF protein anchored cells could elicit a protective and systemtic antitumor responses.
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Clc Number:
R737.9
