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Induction of Human Cervix Cancer Cell Apoptosis by Triptolide
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Abstract:
Objective: To investigate the proliferation inhibitory effect and the apoptosis-inducing activity of triptolide on HeLa cells. Methods: The effects of triptolide on cell proliferation were observed in vitro by methyl thiazolyl tetrazolium (MTT) method. The morphological alterations were confirmed by Hoechst 33258 staining. The apoptosis was evaluated by flow cytometry analysis and agarose gel electrophoresis. Results: Triptolide exhibited a marked antiproliferative effect on HeLa cells. Triptolide induced apoptosis of the cultured cells in a time- and dose-dependent manner, as evidenced by an increased percentage of annexin V/PI positive cells. Correspondingly, we observed a decrease of R123 uptake in Triptolide-treated cells, indicative of a reduction in mitochondrial membrane potential. The morphological changes of apoptotic cells and ladder pattern of DNA were observed in triptolide-treated cells. Conclusion: Triptolide can inhibit the proliferation of HeLa cells by induction of cells apoptosis.
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Clc Number:
R730.59
