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Construction of Prokarytoic Expression Vector of TRAIL Gene and Preparation of Anti-TRAIL Antibody
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Abstract:
Objective:To obtain the anti-TRAIL antibody. Methods:A prokaryotic expression vector pPre-TMHTb was constructed by PCR and recombinant DNA techniques and was confirmed by enzymatic digestion and sequence analysis. TRAIL protein induced to express by IPTG in E.coli DH-5α. After TRAIL protein immunised rabbits, anti-TRAIL antibody was tested by Dot blot and Western blot. Results:A prokaryotic expression vector pPre-TMHTb was constructed and expressd in E.coli DH-5α. TRAIL protein was detected up to 11.8% of the total bacterial protein expressed and anti-TRAIL antibody was tested by Dot blot and Western blot. Conclusions: The anti-TRAIL antibody was obtained that provides an experimental basis for the studies of TRAIL in eukaryotic level.
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Clc Number:
R282 R730.52
