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Induction of Human Cervix Cancer Cell Apoptosis by Triptolide
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Abstract:
Objective: To investigate the proliferation inhibitory effect and the apoptosis-inducing activity of triptolide on HeLa cells. Methods: The effects of triptolide on cell proliferation were observed in vitro by methyl ihiazolyl tetrazoli-um ( MTT) method. The morphological alterations were confirmed by Hoechst 33258 staining. The apoptosis was evaluated by flow cytometry analysis and agarose gel electrophoresis. Results: Triptolide exhibited a marked antiproliferative effect on HeLa cells. Triptolide induced apoptosis of the cultured cells in a time- and dose-dependent manner, as evidenced by an increased percentage of annexin V/PI positive cells. Correspondingly, we observed a decrease of R123 uptake in Triptolide-treated cells, indicative of a reduction in mitochondrial membrane potential. The morphological changes of apoptotic cells and ladder pattern of DNA were observed in triptolide-treated cells. Conclusion: Triptolide can inhibit the proliferation of HeLa cells by induction of cells apoptosis.
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Clc Number:
R373.33
