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Anti-Tumor Effects of Adoptively Transferred Splenocytes Induced by ehCGβ Gene Immunization
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Abstract:
Objective：To investigate the anti-tumor effects of adoptively transferred splenocytes induced by gene immunization with ehCGβ tumor vaccine. Methods：Sp2/0 cells were transfected with the plasmids containing ehCGβ or HBV-preS2/S and the postive clones were screened by G418. BALB/c mice were immunized with plasmid TR421-hCGβ or mock DNA three times. Two weeks after immunization, spleen cells from the immunized mice were harvested, and then analyze the CTL activity against Sp2/0-ehCGβ cells. The splenocytes derived from the immunized mice were adoptively transferred to the normal mice, which were subsequently given injections i.p. of Sp2/0-ehCGβ and β Sp2/0-preS2/S respectively. Results：Splenocytes derived from the mice immunized with TR421-hCGβ exhibited a strong specfic cytotoxic activity against Sp2/0-ehCGβ cells, The average tumor weight between test and control group is statistically significant (P<0.05). Only two mice out of six had tumors in Sp2/0-ehCGβadoptively transferred group, while all mice had tumors in control group. However, all mice challenged with Sp2/0-preS2/S formed tumors, and the average tumor weight was not obviously varied between the two groups. Conclusion：ehCGβ gene vaccine could induce specific cytotoxic splenocytes against ehCGβ and adoptive transfer of the splenocytes showed the anti-tumor activity.
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