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Enhanced Antitumor Effects Induced by MIP-1β Gene-Modified Dendritic Cells Pulsed with Tumor Associated Antigen
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Abstract:
Objective:To probe the potent effects of dendritic cells (DCs)-based vaccine by enhancing the mutual action between DCs and T lymphocytes. Methods: Mouse bone marrow DCs transduced with human Macrophage Inflammatory Protein-1 beta (MIP-1β) gene by adenovirus vector (MIP-1β-DC) were pulsed with murine CT26 colorectal adenocarcinoma cell antigen, and used to vaccinate syngeneic mice.Results: Immunization with CT26 cell antigen-pulsed MIP-1β-DC induced specific CTL against CT26 cells and induced protective antitumor immunity, which rendered the immunized mice resistant completely to CT26 tumor challenge. The result of the study on antitumor immunity mechanism showed that the antitumor response depended on both CD8+ T cells and CD4+ T cells ,which played important roles,while NK cells were not necessary.Conclusions:MIP-1β gene modified DCs are more potent in induction of antitumor immunity through the preferential chemotaxis of DCs on T cells. Vaccination with tumor antigen-pulsed MIP-1β-DC may be a novel approach to immunotherapy of cancer.
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