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Induction of Apoptosis in U343 Cell Line by a Novel Anti-Human DR5 Monoclonal Antibody
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Abstract:
Objective: To investigate the inhibitory effects and mechanism of a novel anti-human DR5(death receptor 5 of TRAIL) monoclonal antibody to glioma cell lines U343.Methods:DR5 protein was tested quantitative through FCM; DR5 mRNA was observed through RT-PCR and distribution was tested by immunocytochemistry. Inhibitory effects and inducing apoptosis of anti human DR5 monoclonal antibody to U343 were analysed by MTT, DNA Ladder,FCM. Results: The expression of death receptor 5 (DR5) was certificated in U343,DR5 appeared to be located in intracellular perinuclear compartment. Inhibitory effects of anti-human DR5 monoclonal antibody on U343 were achieved by 3 μg/ml at 4 hours and the mechanism was associated with apoptosis. Conclusion: Apoptosis of glioma cell lines U343 can be induced by anti-human DR5 monoclonal antibody, and targeted on DR will provide new way to treating cancer.
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