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Activity of TRAIL Gene Modification Combined with DOX on Human Colon Cancer Cell Line RKO
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Abstract:
Objective:To evaluate the gene therapeutic efficiency of TRAIL combined with DOX on human colon cancer cell line RKO. Methods:Human colon cancer cell line RKO was transfected with adenovirus-mediated TRAIL gene Ad/GT-TRAIL and TRAIL/DOX combination. Cell growth and apoptosis were measured by MTT method and flow cytometry. TRAIL gene expression before and after combination with DOX was measured by RT-PCR.Results: The cell line RKO was slightly suppressed by mediate adenovirus and significantly suppressed by TRAIL gene. The suppression percentages were 11.9% and 50.1% respectively. Combined with DOX, the suppression and the apoptosis of cell line RKO could be significantly enhanced by TRAIL gene. The suppression and the apoptic percentage reached 60.3% and 49.0%respectively. RT-PCR showed the expression of TRAIL gene was not enhanced in the RKO cells exposed to the DOX/TRAIL combination.Conclusion: TRAIL gene was able to induce apoptosis and suppress the growth of human colon cancer cell line RKO. Combined with DOX, the suppression and apoptosis could be significantly enhanced. But this result came out not by increasing the expression of TRAIL gene.
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