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Enhancement of Killer Activity of Immune Effector Cells Caused by HLA-G Antisense Oligodeoxynucleotides
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Abstract:
Objective: To explore the mechanism of HLA-G antisense oligodeoxynucleotides (ASODN) on reversion of tumour immune evasion and enhancement of killer activity of immune effector cells. Methods: The expression of HLA-G in JEG-3 cell line was studied by RT-PCR and flow cytometry. MTT assay was used in our studies to detect the change of NK and CD3AK killer activity after ASODN treatment. The effect of IFN-γ secreted by CD3AK after coculture of tumour-lymphocytes was explored by ELISA.Results： HLA-G ASODN could obviously inhibit the expression of HLA-G mRNA and protein. The supernatant of JEG-3 treated by ASODN could reverse the inhibiton of soluble HLA-G molecules to NK killer activity. On the other hand, HLA-G ASODN also could reverse the inhibition of HLA-G to the production of IFN-γ secreted by CD3AK and enhanced the proliferation of CD3AK. Meanwhile, it also improved the susceptibility of JEG-3 to CD3AK.Conclusion:HLA-G ASODN could inhibits the expression of HLA-G mRNA and protein in tumour cells and partly restore the cytotoxicity of NK and CD3AK cells, and thereby partly reverse immune evasion of tumour cells caused by souble HLA-G molecules.
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