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GVHD in Activated Half-Matched Mixed Bone Marrow Transplantation in Experimental Animal Models
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Abstract:
Objective:To observe graft-versus-host disease (GVHD) reaction in activated half-matched mixed bone marrow transplantation (BMT). Methods: Mice 615 with acute radiation disease were used as recipient mice model, with 615C57BL/6 hybrid F1 mice being half-matched donor mice. Half-matched murine bone marrow and spleen cells mixed with certain percent of syngeneic spleen cells were activated in vitro with IL-2 and underwent mixed half-matched BMT to observe their death rate, hematopoietic recovery of myelocytic lineage, spleen node count, mixed lymphocyte reaction in vivo, chimerism and pathological changes, to compared with GVHD reactions of various transplant models. Results:GVHD was relatively severe in both activated and non-actived half-matched BMT groups, showing that activated half-matched mixed 3∶1 BMT group after activation could not alleviate GVHD, whereas the activated half-matched mixed 1∶1 BMT group or 2∶1 group with high percentage of syngeneic spleen cells could lessen the extent of GVHD, as manifested by the quick recovery of peripheral blood WBC count and bone marrow GM-CFU formation, by the low of mixed lymphocyte reaction in vivo as well as by the increase of chimeric rate. Conclusions:Half-matched mixed BMT after activation could lower the GVHD reaction, which was relative to the ratio of syngeneic to allogeneic spleen cells.
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