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Inhibition of Transplantable Tumor of Nasopharyngeal Carcinoma Cells Line by Specific LMP2-CTLs
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Abstract:
Objective：To investigate inhibitory effect of specific cytotoxicity lymphocytes(CTLs) pulsed by EBV-LMP2-peptide epitopes on transplantable tumor of nasopharyngeal carcinoma cell (NPC)line-CNE2 with identical HLA-Ⅰ molecule subtypes, and explore the possibility of immunotherapy among indivduals. Methods：Transplantation tumor was established through subcutaneous injection of CNE2 cells in Scid mice. HLA-Ⅰ subtypes in CNE2 cells were identified by specific site primer polymerase chain reaction (SSP-PCR). Matched HLA HLA-Ⅰmolecule subtypes with CNE2 were screened among normal volunteers. Specific CTLs stimulated by EBV-LMP2-peptide epitopes were collected for the next steps. Three groups were divided. (1) CTL group: CNE2 cells with specific CTLs stimulated by EBV-LMP2-peptide epitopes were injected into mice subcutaneousness. (2) T group: CNE2 cells with t lymphocytes were injected. (3) The control group, CNE2 cells were injected only. The results were observed in a few weeks later. Results：Transplantable tumors were observed after CNE2 having been injected into scids mice. HLA-A11 in CNE2 cells was detected by SSP-PCR. Comparing the results from the three groups, we found specific CTLs stimulated by A11-LMP2-pepetide epitopes distinctly retarded the growth of CNE2 transplantable tumor. Conclusion：Specific-LMP2 CTLs with HLA-A11 molecule subtype effectively inhibit the development of transplantable tumor of CNE2 cells with identical HLA-Ⅰ molecule subtype of three different groups were injected.
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