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Anti-Tumor Efficacy Induced by Antibodized PDTRP Gene Immunization
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Abstract:
Objective: To study the anti-tumor efficacy induced by antibodized tumor epitope PDTRP gene immunization. Methods:Three copies of tumor associate gene PDTRP from MUC1 tandem repeats were designed and mimicked the conformation of MUC1 by Insight Ⅱ. The γ1neo-PDTRP plasmid was further constructed, in which the PDTRP target gene was inserted into CDR3 of the γ1-neo vector. The specific humoral and cellular immune responses towards to PDTRP were detected after intraspleen immunized Balb/c mice with γ1neo-PDTRP. And the immune protection assay was also done to observe whether the mice immunized with γ1neo-PDTRP could prolong the survival after tumor challenge. Results: The conformation of three copies of PDTRP mimicked the conformation of MUC1 tandem repeats. The expression of γ1neo-PDTRP could be detected after in vitro transfect. The specific antibody against PDTRP epitope could be induced and increase to a higher titer after intraspleen injection with a γ1neo-PDTRP plasmid. And the specific proliferation and cytotoxic function of lymphocyte were also increased. There is a significant survival from mice immunized with γ1neo-PDTRP against the 4T1-PDTRP tumor challenge.Conclusions: Gene immunization with γ1neo-PDTRP could elicit both humoral and cellular tumor specific immune response and had the protective effect.
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