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Clonging and Expression of Recombinant Human Vasostatin’s Domain and Its Ability to Inhibit Angiogenesis in CAM
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Abstract:
Objective: To clone and express the recombinant human Vasostatin120-180 aa domain and to investigate its activity of inhibiting angiogenesis in CAM. Methods:After amplifying gene of human Vasostatin120-180 aa domain, we subcloned it into pQE30 vector and expressed Vasostatin120-180 aa domain by E.coli. We also tested its ability of inhibiting angiogenesis in CAM. Results: The total gene length of human Vasostatin120-180 aa domain is 180 bp. Expressed by pQE30 system in E.coli and purified by IMAC, Vasostatin120-180 aa was detected by SDS-PAGE, in which there is a positive band and molecular weight is about 8 kD.Conclusions:Recombinant human Vasostatin120-180aa could play effective role in anti-angiogenesis in CAM and it showed a dose dependent effect in some degree.
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