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Effects of Antisense Oligoxydeonucleotides Targeting the mRNA of Ki67 Gene on the Proliferation and Apoptosis of Human Renal Carcinoma Cells
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Abstract:
Objective:To investigate the effects of antisense oligoxydeonucleotides of Ki67 gene(Ki67-ASODNs) on the proliferation and apoptosis of human renal carcinoma cell line 786-0 cells. Methods: Human renal carcinoma cell line 786-0 cells were treated with Ki67-ASODNs (10, 40 μmol/L).The Ki67 expression of 786-0 cells was detected by immunohistochemical technique and Western blot respectively. The proliferation of 786-0 cells was studied by cell growth curves and 3H-TdR uptade assay. The apoptosis of 786-0 cells was detected by TUNEL assay. Results: The Ki67 expression rates of 786-0 cells treated by Ki67-ASODNs （29.9±0.4, 24.5±1.2）were lower than that of the control group(P<001). The Ki67 protein quantity rates of 786-0 cells treated by Ki67-ASODNs （82.1±2.2, 66.6±4.2）were reduced markedly(P<0.01). The 3H-thymidine incorporation rates of 786-0 cells treated by Ki67-ASODNs（44.5±4.4, 34.5±3.2） were lower than that of the control group(P<0.01).The apoptosis rates of 786-0 cells treated by Ki67 ASODNs（12.7±0.5, 23.1±2.1）were higher than that of the control group（9.3±2.0； P<0.01). Conclusions: Antisense oligoxydeonucleotides of Ki67 could inhibit the proliferation and induce apoptosis by blocking Ki67 expression of human renal carcinoma 786-0 cells.
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