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Clonal Expansion and Specific Cytotoxicity of TCR Vβ Subfamily T Cells Induced by AML-M2a Cells
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Abstract:
Objective: To investigate the clonal expansion and specific cytotoxicity of TCR Vβ subfamily T cells from healthy individuals induced by acute myelogenous leukemia M2a subtype (AML-M2a) cells, respectively.Methods: T cells from three healthy individuals that were induced by AML-M2a cells by using mixed lymphocyte and tumor culture (MLTC) in different amplified stage were detected for the specific cytotoxicity by LDH release assay. The distribution and clonal expansion of TCR Vβ 24 subfamily genes of T cells before and after induction were analyzed by RT-PCR and genescan.Results: At 12th and 21st day after culture the cytotoxicity rates of three healthy individuals T cells after MLTC for the induced cells. (AML-M2a cells) were 39.88±7.79% and 62.14±9.79%, respectively, whereas there were not any cytotoxicity in the T cells of peripheral blood from the AML-M2a case and three healthy individuals. Clonal expansion of TCR Vβ16 subfamily T cells was displayed by genescan in the T cells from three donors after MLTC.Conclusions: The clonal expansion and specific cytotoxicity T cells from healthy individuals could be induced by AML-M2a cells. The clonal expansion T cells were more tended to TCR Vβ16 subfamily.
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