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ntitumor Effect of Recombinant NDRG2 Protein on Tumor Cells
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Abstract:
Objective: To study the effects of prokaryotically expressed recombinant human NDRG2 on tumor cell lines. MethodsRecombinant human NDRG2′s inhibitory effects on cell lines 7901 and HHCC at a range of protein concentrations were measured by MTT colorimeteric assay and cell counting against control groups. In the present study, the cell cycle changes were assessed by flow cytometry, and in vivo tumor inhibition study was performed on nude mice. Results: Recombinant human NDRG2 exerted strong inhibitory effects on tumor cell growth, and started to significantly suppress tumor cell growth at the concentration of about 50 μg/ml. Flow cytometry analysis indicated that such inhibition triggered changes to the G1 phase in the tumor cell cycle. In addition, the growth of the tumor xenografts in nude mice was signficantly suppressed in the in vivo study. Conclusion: Recombinant human NDRG2 exhibits cdertain inhibitory effects on tumor cell growth both in vivo and in vivo, which presents an interesting finding that will offers us insight into the cancer treatment.
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