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The Expressive Detection for RFP Protein in Human, Mouse Normal Tissues and Human Esophageal Squamous Cell Carcinoma
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Abstract:
Objective: To investigate the distribution of RFP( Ret Finger Protein) in Human, mouse normal tissues and human esophageal squamous cell carcinoma. Methods: Real-time Quantitative RT-PCR with 18SrRNA as the internal standard was used to detect the expression of RFP in human normal tissues such as cervical tissue, endometrial tissue, testis tissue, gastric tissue, esophageal tissue, brain tissue, human esophageal squamous cell carcinoma and mouse normal tissues such as lung tissue, kidney tissue, uterus tissue, ovary tissue, thymus tissue, heart tissue, muscle tissue, testis tissue, skin tissue, esophageal tissue, spleen tissue, liver tissue, colon tissue, intestinum tissue. Results: The highest level of RFP mRNA was only present in testis tissue among human and mouse normal tissues. In addition, the up-regulation of RFP protein was observed in 13 out of 20 human esophageal squamous cell carcinoma tissues, but only the upregulation of RFP in 9 out of 20 is significant（P<0.05）. Conclusions:Regarding to the expression of RFP limited to testis among human and mouse normal tissues and the up-regulation of RFP in 13 out of 20 human esophageal squamous cell carcinoma tissues, the RFP can be used as a potential molecular target for the development of new therapeutics for human esophageal squamous cell carcinoma.
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