Mobile website
Inhibition of survivin and bcl-2 Antisense Oligodeoxynucleotides Combined Transfection on the Human Gallbladder Carcinoma Cell Line GBC-SD in vitro
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective:To investigate the effect of survivin and bcl-2 antisense oligodeoxynucleotides (AsODN) combined transfection on the human gallbladder carcinoma cell line GBC-SD in vitro.Methods: Survivin and Bcl-2 protein expressions were detected by immunohistochemical method; Cultured cells were divided into 4 groups: Nomal control group, survivin antisense observed group, bcl-2 antisense observed group and combined group. After transfected for 24 h, the expression of survivin mRNA was detected by reverse transcription polymerase chain reaction(RT-PCR). Cell morphological changes were observed under electron microscopy. Apoptosis index (AI) was examined by flow cytometry; Inhibitory rate (IR ) was determined by the colorimetri MTT cell viability and proliferation assay.Results:Survivin and Bcl-2 protein were highly expressed in gall bladder carcinoma cells; The expression of survivin mRNA was decreased 47.8%. Abnormal morphological changes of cells were observed in the three AsODN transfection groups; The AI in survivin antisense observed group,bcl-2 antisense observed group,and combined group was 11.38％±3.91％, 9.26％±4.15％, 28.45％±6.34％ respectively and significantly higher than the nomal control group (P<0.01), and that in the combined group was significantly higher than the other two observed groups(P<0.05). The IR was 54.3％,47.6％,76.5％ respectively in the three AsODN transfection groups. Conclusion:Survivin and bcl-2 protein were highly expressed in gallbladder carcinoma cells. Survivin AsODN and bcl-2 AsODN can induce GBC-SD cell apoptosis and the effect was more markedly by combining the two AsODNs.
Keywords:
Project Supported:
Clc Number:
