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Regulation of Chk1/2 on Apoptosis of Cancer Cells
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Abstract:
Objective: To investigate the cell cycle change of K562 cells and bone marrow mononucleate cells(BMMNC) of leukemic patient induced by DDP and the role of antisense oligonucleotide targeting Chk1/2.Methods: The change of cell cycle was assayed by means of flow cytometer after different interval in which the K562 cells and BMMNC of leukemia are treated by DDP. K562 cells or BMMNC are transfected with the antisense oligonucleotide complementary to Chk1/2 by lipofection. The apoptosis of K562 cells and BMMNC induced by DDP was investigated by flow cytometer after transfection. Results: K562 cells and BMMNC of leukemia arrested at S phase at 10μmol/L of DDP. The antisense oligonucleotide targeting Chk1 or Chk2 could increase the apoptosis of K562 cells treated with DDP. The antisense oligonucleotide targeting Chk1 could increase the apoptosis of leukemic BMMNC treated with DDP, but the antisense oligonucleotide targeting Chk2 could not increase the apoptosis of BMMNC of leukemia induced by DDP.Conclusion: Chk1 may take part in apoptosis regulation of leukemia cells and be used as target of leukemia therapy.
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