Mobile website
The Anti-Lung Cancer Effects of Canstatin Recombinant Vector
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective:To construct a mammal expression system of human canstatin and study its anti-tumor effects on lung cancer. Methods:Canstatin cDNA was acquired by RT-PCR, and cloned into a mammal exprssion vector named pCMV-Script. Canstatin expression was detected by Real-time PCR. The proliferation, apotosis of the cells transfected with recombinant canstatin vector were measured by trypan blue exclusive assay, 3H-thymidine incorporation and TUNEL method respectively. Then the recombinant vector encoding canstatin cDNAs was transferred into tumors of cancer-bearing nude mice with electroporator in vivo, and micro-vessel count was proceeded of each tumor by anti-CD31 antibody immunohistochemical staining.Results: The recombinant vector pCMV-Script-Cans was successfully constructed , and the canstatin mRNA was detected in both of the transformed HUVE and A549 cells. The 3H-TdR intake rate in pCMV-Script-Cans transformed HUVE cells is significant lower than that of the naked plasmid transformed cells (P<0.001) , while the apotosis rate of them is significant higher than that of the control cells(P<0.001). The micro-vessels in the recombinant vector transformed tumors were significant lower than that of the control group. Conclusions: Canstatin only inhibit cell proliferation and induce apotosis in endothelial cell, and it also has a good anti-tumor effect in vivo.
Keywords:
Project Supported:
Clc Number:
