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Construction of Attenuated Salmonella Typhimurium Carrying flk1 Expression Vector and Priliminary Investigation of Its Inhibition of Tumor Angiogenesis
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Abstract:
Objective : To investigate the specific anti-flk1 immunity and the anti-angiogenesis effect of attenuated Salmonella typhimurium strain carrying flk1 expression vector encoding flk1 cDNA. Methods：Plasmid pcDNA3.1-flk1 was constructed and transformed into live attenuated Salmonella typhimurium strain SL7207. The recombinant bacteria were used to infect murine macrophage in vitro and the expressed products were detected by Western blot. C57BL/6J mice were immunized with recombinant bacteria encoding flk1, and CTL activity of the splenocytes derived from the immunized mice was measured by MTT assay. Alginate bead assay was designed to measure in vivo angiogenesis induced by tumor cells. Result: Murine macrophage infected with recombinant S. typhimurium transformed with plasmid pcDNA3.1-flk1 could express flk1 protein. A strong CTL activity against flk1 was found in pcDNA3.1-flk1 immunized animals. The recombinant S. typhimurium vaccination inhibited tumor-induced angiogenesis in vivo. Conclusion: Attenuated Salmonella typhimurium strain encoding flk1 cDNA can elicit specific cellular immune response and inhibit tumor-induced angiogenesis in vivo.
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