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The Inhibition of Antisense TRP1 in the Proliferation of Malignant Melanoma Cells in vivo and in vitro
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Abstract:
Objective：To study the inhibition of antisense TRP 1 on cell growth of malignant melanoma(MM) and explore a new way for therapy of melanoma.Methods：Antisense TRP-1 recombinant vector was constructed and transfected into MM cells. According to the results of MTT, cell growth curves were drawn and then clonogenic assay was performed in vitro. At last, tumorigenesis assay was undertaken in nude mice in vivo.Results：Cell proliferations of TRP-1 transfected MM cells were inhibited compared with the control cells. The results of clonogenic assay displayed the difference of clonogenic percentage between TRP-1 transfected MM cells (52%, P<0.01) and the control cells (68%, P<0.01). Tumorigenesis assay in vivo 30 days after implantation showed that the growth of TRP-1 antisense vector transfected MM cells was significantly suppressed. Conclusion：Antisense TRP-1could inhibit the proliferation of MM cells both in vivo and in vitro. It could be expected that antisense TRP-1 be used in the therapy of MM in the future.
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