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The Therapeutic Effect of Combination of the Human Papillomavirus 16 (HPV16) E6/E7 Fusion Protein Vaccine Combined with Radiotherapy on Cervical Cancer
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Abstract:
Objective: To evaluate the therapeutic effect of combination of the human papillomavirus 16 (HPV16) E6/E7 fusion protein vaccine with radiotherapy on the HPV16-positive cervical cancer. Methods: Twenty-four female C57BL/6 mice 7 days after inoculation of tumor cells on the right hind legs were randomly divided into four groups: The control group (C, n=6); Immunotherapy group (IM, n=6); Radiotherapy group (RA, n=6); Immunotherapy+Radiotherapy (IM+RA, n=6). The growth rates of tumors and survival time were recorded and compared with each other. The apoptotic tumor cells in situ were detected by TUNEL assay. Results:The growth rates of tumors in IM+RA- group were lower than other groups. The mean survival time in IM+RA-group was longer than those in IM-, RA- and C-groups, but the difference between IM- and C- group was not significant. The result of TUNEL assay indicated that there were more apoptotic tumor cells in IM+RA- group than that in other groups. Conclusion:The combination of HPV16E6/E7 fusion protein vaccine with low dose radiotherapy exerted synergic antitumor efficacy, which might provide a new approach for the treatment of cervical cancer.
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Clc Number:
R511 R392-33
