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Effect of TNF-Related Apoptosis Inducing Ligand on Hepatocellular Carcinoma in Mice
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Abstract:
Objective：To evaluate the sensibility of apoptosis to TRAIL on the residual H22 tumor cells after chemotherapeutic agents treatment and the synergic therapeutic efficiency of pCH510, pTRAIL, and pES in combination with chemotherapeutic agent on mice tumor. Methods：Carrying full length of TRAIL gene, the eukaryotic expression plasmid of pTRAIL was transferred into BHK cells. The mouse hepatocellular carcinoma cell line of H22 which had been treated with ADM, MMC, or 5-FU were mixed with BHK cells. The inhibitory effect of pTRAIL in combination with chemotherapeutic agents was detected by MTT method. The percentage of apoptotic cells and cell cycle of residual H22 cells were analyzed by flow cytometry. The tumor model was made by inoculated with H22 hepatocarcinoma cells in mice. After injection of pCH510, pTRAIL, pES or MMC into intratumor, The therapeutic effects on tumor growth were assessed. Results: pTRAIL could inhibit the growth of H22 tumor cells and induce them to apoptosis. The percentage of apoptotic cells of TRAIL in combination with ADM, MMC, or 5-FU on H22 tumor cells was 28.1%（P<0.01）, 22.5% (P<0.01), and 47% (P>0.05). The tumor was effectively inhibited by MMC+pCH510+pTRAIL+pES . And the percentage of tumorogenesis was 37.5%. Residual tumor cells were scattered and mixed with immune cells from histological detection. Conclusion: Residual H22 tumor cells after ADM or MMC treatment is apt to be induced to apoptosis by pTRAIL. MMC +pCH510+pTRAIL+pES is a great powerful modality for tumor synergic treatment.
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Clc Number:
R378.71 R392-33
